Extended Data Fig. 8: Compromised GC formation and output and retarded affinity maturation in the absence of T cell CCR4.
From: Affinity-coupled CCL22 promotes positive selection in germinal centres
Analyses of GCs and BMPCs in Tcrb−/−Tcrd−/− (TCR-KO) bone marrow chimaeric mice. a, b, Representative contour plots of GC B cells (a) and BMPCs (b) of TCR-KO (80%):wild-type (20%) or TCR-KO (80%):CCR4-KO(20%) bone marrow chimaera. c, d, Summary statistics of GC B cells (c) and BMPCs (d) at indicated time points after immunization. Each dot indicates one chimaeric mouse, and lines denote means. Data are pooled from three independent experiments. P values by two-tailed t-tests. e, f, Numbers of W33L mutations among unique VH186.2 sequences of GC B cells (e) and BMPCs (f) at indicated time points. Total numbers of VH186.2 sequences analysed are given at the centre of each pie chart, and total numbers of nucleotide mutations per sequence are given under each pie chart as mean ± s.e.m. Data are pooled from three independent experiments. P values by two-tailed Fisher’s exact tests.
