Extended Data Fig. 10: The CCR4–CCL22 axis in human TFH and GC B cells.
From: Affinity-coupled CCL22 promotes positive selection in germinal centres
a, Representative gating of non-TFH cells, CXCR5+ T cells and CXCR5+PD1+ tonsillar TFH cells. b, Histograms of CCR4 expression overlaid with isotype control staining (iso). c, CCR4 mean fluorescent intensities (MFI), with respective isotype control background subtracted. Data are mean ± s.e.m. of four donors. P values by two-tailed t-tests. d, Representative gating of human tonsillar GC B cells (left), further divided into light-zone (LZ) and dark-zone (DZ) fractions (right). e, CCL22 upregulation by light-zone GC B cells after 4 h anti-CD40 or control hamster IgG treatment. Data are pooled from nine independent donors. P values by two-tailed Mann–Whitney U-tests. f, Violin plots of CD40 signature genes, identified by Holmes et al.15, as probed by scRNA-seq. The top and bottom rows present cells characterized with CCL22 expression >0 and =0, respectively. Single-cell data were collected from three healthy donors. P values were calculated on the basis of hypergeometric distribution (see Methods for details).
