Extended Data Fig. 3: Schemes for genetic ablation of Ccl22, Ccl17 and Ccr4.

a, b, CRISPR–Cas9-mediated strategy for genomic deletion of Ccr4. The targeting sgRNA (red line), genotyping primers (yellow arrowheads) and the deleted segment (yellow highlight) are shown in the context of genomic sequences (nucleotide coordinates according to NCBI) (a), with representative genotyping PCR products (b), which differ between wild-type and Ccr4-KO alleles by 8 bp and are distinguished by sequencing. c, Surface CCR4 expression on CD4⁺ T cells of indicated genotypes following in vitro activation with anti-CD3 and anti-CD28 for 4 days. d–g, CRISPR–Cas9 strategies for Ccl17 and Ccl22 ablation. Relevant targeting sgRNAs (red lines), genotyping primers (yellow arrowheads) and deleted segments (purple lines) are shown in the context of genomic sequences (nucleotide coordinates according to NCBI) for Ccl17 (d) and Ccl22 (f), with representative genotyping PCR results shown for Ccl17 (e) and Ccl22 (g).