Extended Data Fig. 1: Human–mouse PSC co-culture.
From: Cell competition constitutes a barrier for interspecies chimerism
a, Representative brightfield images of H9-hES cells (passage 51) and mEpiSCs (passage 30), in the F/R1 culture condition. Scale bar, 200 μm. b, Representative immunofluorescence images of mEpiSCs (passage 32), H9-hES cells (passage 49), H1-hES cells (passage 51) and HFF-hiPS cells (passage 21), in the F/R1 culture condition, expressing pluripotency transcription factors SOX2 (green) and OCT4 (red). Blue, DAPI. Scale bars, 200 μm. c, Long-term F/R1-cultured H9-hES cells (passage 49) and HFF-hiPS cells (passage 23) exhibited normal karyotypes. d, Flow cytometry analysis of cell cycle phase distribution of H9-hES cells and mEpiSCs after 3 days in separate and co-cultures. n = 3, biological replicates. Data are mean ± s.e.m. e, H9-hES cells maintained the expression of pluripotency markers OCT4, SOX2 and TRA-1-60 after 3 days of separate and co-cultures. f, mEpiSCs maintained the expression of pluripotency markers CD24, SOX2, SSEA1 and OCT4 after 3 days of separate and co-cultures. Images in a and b are representative of three independent experiments.
