Extended Data Fig. 1: Spo11 and Rec8 cause meiotic KT loss in the absence of HC.
From: Centromeres are dismantled by foundational meiotic proteins Spo11 and Rec8
Quantification of KT loss phenotypes as exemplified in Fig. 1a. a–c, KTs are visualized with Ndc80-2xGFP (a) or Cnp3CenpC-GFP (b, c). spo11Δ and rec8Δ rescue KT loss in clr4Δ cells while deletions in other subcomplexes25 of the meiotic recombination machinery do not (a). spo11+ deletion or the DNA binding-deficient spo11-dbd mutant rescue KT loss in the absence of Swi6HP1, while spo11-cd triggers KT loss as does wt spo11 (b). rec8+ deletion, but not rec11+ deletion, suppresses KT loss in the absence of Swi6HP1, mirroring these proteins’ effects in bqt1Δ cells (c). Total numbers of films analysed are indicated above each bar. P values are determined by two-tailed Fisher’s exact tests and indicated above brackets.
