Extended Data Fig. 2: Overexpression of Spo11 or Rec8 elevates KT loss rate, and chromatin remodellers are required for meiotic KT dismantlement.
From: Centromeres are dismantled by foundational meiotic proteins Spo11 and Rec8
Quantification of KT loss phenotypes as in Fig. 1a. a, Expressing a tandem copy of spo11+ under the control of weaker (relative to adh1 or nmt41) adh13 promoter (spo11.OE3) does not confer KT loss in a bqt1+ setting, but increases KT loss in the bqt1Δ setting. b, c, Strong overexpression of spo11+ (spo11.OE1, controlled by nmt41 promoter) or rec8+ (rec8.OE, controlled by adh1 promoter) elevates the frequency of meiotic KT loss in bqt1Δ (b) and swi6Δ (c) settings. Total numbers of films analysed indicated above each bar. P values are determined by two-tailed Fisher’s exact tests and indicated above brackets. d, Loss of a CHD remodeller (Hrp3) or RSC component (Rsc1) rescues KT loss in the absence of swi6HP1; other chromatin remodellers have no effect. Total numbers of films analysed indicated above each bar. P values are determined by two-tailed Fisher’s exact tests and indicated above brackets.
