Extended Data Fig. 3: RSC facilitates Rec8 localization to meiotic centromeres.
From: Centromeres are dismantled by foundational meiotic proteins Spo11 and Rec8
a, Frames of representative films monitoring endogenously GFP-tagged Rec8 through meiosis are shown. During prophase, Rec8 localizes throughout the nucleus. As cells approach MI, Rec8 begins to disappear but remains concentrated at centromeres, as seen at enhanced brightness. At the end of MI, Rec8 foci are clearly visible in the wt cell but not in the rsc1Δ cell. The frames highlighted in the yellow box are shown below; these foci subsequently disappear at MII. Scale bars, 5 μm. b, Quantification of meiotic cells harbouring zero, one or two Rec8 foci at the end of MI. Total numbers of films analysed are indicated above each bar. All rsc1Δ films show diminished Rec8-GFP at the end of MI, and most of them fail to show any detectable Rec8-GFP on at least one SPB.
