Extended Data Fig. 1: Human epithelial cells in the human lung tissue of LoM express ACE2.
From: SARS-CoV-2 infection is effectively treated and prevented by EIDD-2801
a, H&E staining of the human lung tissue of a naive LoM (n = 6). Boxes in the left panel indicate regions that are shown in higher-magnification images in the right panels, of cartilaginous airways (top) and noncartilaginous airways and alveoli (bottom). Scale bars, 500 μm (left), 100 μm (top right, bottom right). b, Immunofluorescence staining for human cytokeratin 19 (epithelial cells, green; nuclei, blue), CD34 (endothelial cells, green; nuclei, blue) or vimentin (mesenchymal cells, green; nuclei, blue) (n = 8). Scale bars, 50 μm. c, Immunofluorescence staining for human ACE2 in the human lung tissue of a naive LoM (positive cells, red; nuclei, blue) (n = 9). Scale bar, 50 μm. d, Costaining for human ACE2 (positive cells, red) and cytokeratin 19 (positive cells, green) in human lung tissue from naive LoM (nuclei, blue) (n = 9). Scale bars, 50 μm. e, Immunohistochemical staining for TMPRSS2 in human lung tissue from naive LoM (positive cells, brown) (n = 6). Scale bar, 100 μm. n, number of biologically independent lung tissues analysed.
