Extended Data Fig. 6: Expression dynamics of PTI signalling components during ETIAvrRps4.
From: Mutual potentiation of plant immunity by cell-surface and intracellular receptors
a, Transcript induction of SOBIR1, BAK1, BIK1, RBOHD, MPK3, CERK1, MPK4, MPK6, ICS1 and PR1 during ETIAvrRps4 over 24 h. Transcript levels were normalized to EF1A. Data are mean ±s.e.m. (from three independent experiments). A two-sided Welch’s t-test was used to analyse significant differences of data points from ETIAvrRps4-activated samples compared to untreated samples (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.005; ****P ≤ 0.001; otherwise, not significant). Exact P values are provided in Supplementary Table 5. b, Relative mRNA expression changes of ICS1 (green) and PR1 (black) during ETIAvrRps4. Expression changes of the corresponding genes relative to untreated samples (dotted line shows a log2-transformed fold change of 0) are shown. Solid line, mean; shaded band, s.e.m. c, Heat map representing log2-transformed fold changes in gene expression of transcripts from a (normalized against expression of the corresponding genes in the untreated sample). Gene expression values at the indicated time points are relative to untreated samples. Red, upregulation; blue, downregulation. d, Protein accumulation of PR1 at different time points. Ponceau staining of western blots from Fig. 3b is also shown. e, Serial dilution to estimate protein accumulation of BIK1, RBOHD and MPK3 at 8 h after ETIAvrRps4 activation compared to 0 h. Red asterisk indicates approximate fold differences between 0 h and 8 h. f, Five-week old Arabidopsis Est:AvrRps4 rosette leaves were treated with hrcC−, oestradiol or both (hrcC− + est) for the indicated times and both RNA and proteins were extracted. Extracted RNA was analysed by qPCR and expression level is presented relative to EF1A. Data are mean ±s.e.m. (from three independent experiments; PTI, red; ETIAvrRps4, yellow; PTI + ETIAvrRps4, blue). A two-sided Welch’s t-test was used to analyse significant differences of 4-h and 8-h data points from 0 h (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.005; ****P ≤ 0.001). Exact P values are provided in Supplementary Table 5. For d–f, Ponceau staining was used as loading control. Molecular weight markers (in kDa) are indicated on the left. All experiments were repeated at least three times with similar results.
