Extended Data Fig. 4: mRNA transcript accumulation of PTI signalling components during ETI.
From: Mutual potentiation of plant immunity by cell-surface and intracellular receptors
a, Relative gene expression of BAK1, SOBIR1, BIK1, RBOHD, MPK3, MPK6, FLS2, CERK1, MPK4 and RBOHF relative to EF1A in several effector-inducible lines. Five-week-old leaves of inducible AvrRpm1, AvrRpt2, AvrPphB, AvrRps4 and AvrRpp4 lines were infiltrated with 50 μM dexamethasone (for Dex:AvrRpm1) or 50 μM oestradiol. Samples were collected at 0 h, 4 h and 8 h after infiltration for RNA extraction. b, Heat map representing log2-transformed fold changes in gene expression of BAK1, SOBIR1, BIK1, RBOHD, MPK3, MPK6, FLS2, CERK1, MPK4 and RBOHF from a (normalized against expression of the corresponding genes in sample at 0 h). Gene expression at 4 h and 8 h was normalized to expression level at 0 h. Red, upregulation; blue, downregulation. c, Protein accumulation of BIK1, RBOHD and MPK3 during ETIAvrRps4 is abrogated in eds1-2. Proteins were extracted from Est:AvrRps4 and Est:AvrRps4 eds1-2 after treatment with oestradiol for 0 h, 4 h and 8 h. Molecular weight markers (in kDa) are indicated on the left. Ponceau staining was used as loading control. d, Transcript induction of BIK1, RBOHD and MPK3 during ETIAvrRps4 is abrogated in eds1-2. For a, d, extracted RNA was analysed by qPCR and expression level is presented relative to EF1A. Data are mean ±s.e.m. (from three independent experiments). A two-sided Welch’s t-test was used to analyse significant differences of 4-h and 8-h data points from 0 h (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.005; ****P ≤ 0.001). Exact P values are provided in Supplementary Table 5. All experiments were repeated at least three times with similar results.
