Extended Data Fig. 8: scRNA-seq reveals increased proliferation and inflammatory macrophages.

a, Heat map of top five differentially expressed genes enriched in each cluster. b, Table showing top ten differentially expressed genes in each cluster with cell annotations (each sample pooled from eight mice). c, Cell cycle classification based on cell cycle gene expression using Seurat analysis; one-tailed χ² test, P = 1.415 × 10⁻¹⁰ indicates G2M cells enriched in Jak2^VF lesions. d, UMAP plot of cluster 13 enriched Alox15. Blue indicates relative gene expression. e, Representative immunofluorescence images of aortic root lesions stained for MAC2 (green), ALOX15 (magenta) and DAPI (blue). White dashed lines, plaque; arrows, ALOX15⁺ cells; scale bars, 50 μm. f, Quantification of ALOX15⁺ cells per aortic root section (n = 14 Jak2^VF, n = 13 Jak2^VFGsdmd^−/− mice). Mean ± s.e.m.; *P < 0.05 with respect to control genotype, two-tailed Mann–Whitney test (f).

Source data