Extended Data Fig. 10: PKC isozymes regulate cavity formation in human blastoids.
From: Blastocyst-like structures generated from human pluripotent stem cells
a, Representative immunofluorescence co-staining images of phalloidin and ZO1 in cell aggregates generated at different time points during blastoid formation. Scale bars, 100 μm. b, Representative phase-contrast images of cell aggregates after treatments with DMSO control (top), Gö6976 (middle) or Gö6983 (bottom). Red arrowheads indicate blastoids. Scale bar, 100 μm. c, Counts per million (CPM) values calculated from a published scRNA-seq dataset29, showing the expression levels of different PKC isozymes at different stages during human pre-implantation development. E, early stage; L, late stage. d, e, Representative immunofluorescence co-staining images of ZO1, phalloidin and GATA3 in cell aggregates after treatments with inhibitors of PKCα (d) and PKCζ (e). Higher-magnification images of the boxed areas are shown on the left. Scale bars, 100 μm. Images in a, b, d, e are representative of at least three independent experiments.
