Extended Data Fig. 9: Cryo-EM analysis of heterosubtypic H5 HA in complex with polyclonal antibody Fab fragments prepared from NHP immunized with qsMosaic-I53_dn5.
From: Quadrivalent influenza nanoparticle vaccines induce broad protection
a, Representative cryo-electron micrograph. Scale bar, 100 nm. b, Reference-free 2D class averages. Scale bar, 20 nm. c, Gold-standard FSC curve for the asymmetric reconstruction shown in d. d, Asymmetric cryo-EM reconstruction of H5 HA–Fab complexes with Fab fragments bound to all three HA subunits at 3.6 Å resolution. The reconstruction is the same as that shown in the right panel in Fig. 5d, but here is coloured by local resolution. e, FSC curve for the asymmetric reconstruction shown in f. f, Two orthogonal orientations of an asymmetric cryo-EM reconstruction of H5 HA–Fab complexes with Fab fragments bound to two HA subunits at 4.1 Å resolution. g, FSC curve for the asymmetric reconstruction shown in h. h, Two orthogonal orientations of an asymmetric cryo-EM reconstruction of H5 HA–Fab complexes with Fab fragments bound to one HA subunit at 4.0 Å resolution. The reconstruction is the same as that shown in the left panel in Fig. 5d. All experiments were performed once.
