Extended Data Fig. 8: Gentamicin assay demonstrating the entry of bacteria into melanoma cells.

a, b, Colony-forming units (CFU) of A. odontolyticus and S. caprae after coculture with 51AL and 55A3 melanoma cells. Less-invasive bacteria (L. animalis and L. plantarum) were used as a control, and show lower CFU. c, d, CFU of S. capitis (c) (isolated from tumour 58) after coculture with 58A melanoma cells, or S. succinus (d) (isolated from tumour Mel261) after coculture with 51AL or 55A3 cells. Cells were cultured with the indicated bacteria for 4 and 8 h. ‘Supernatant’ refers to the CFU of medium taken from samples incubated with gentamicin after coculture with the bacteria (grey). ‘No gentamicin’ refers to samples not treated with gentamicin after the coculture (red). ‘With gentamicin’ refers to samples treated with gentamicin for 1 h after the coculture (blue). Bars represent the average of s.e. between biological replicates (n = 3). P values from Student’s t-test between the supernatant sample and the without gentamicin or with gentamicin samples; P values between S. caprae or A. odontolyticus with gentamicin to L. animalis or L. plantarum with gentamicin control samples are from a one‐way analysis of variance followed by Tukey’s test, and are presented in Supplementary Table 4.