Extended Data Fig. 9: Immunofluorescence staining of melanoma cells cocultured with aerobically grown bacteria, demonstrating the ability of the bacteria to enter the cells.
From: Identification of bacteria-derived HLA-bound peptides in melanoma
a, Melanoma cells expressing GFP (green) were cocultured with the aerobically grown bacteria S. caprae, S. capitis or S. succinus stained with antibacterial antibody lipoteichoic acid (LTA) (red); cell nuclei were stained with DAPI (blue). White arrows indicate the location of bacteria that entered the melanoma cells. b, A representative image of 51AL cells expressing GFP (green) cocultured with S. caprae and stained without a primary LTA antibody (red), to exclude nonspecific staining. Images are presented at 63× magnification. Scale bars, 10 μm. Figures are representative of at least three independent experiments.
