Extended Data Fig. 10: Immunofluorescence staining of melanoma cells cocultured with anaerobically grown bacteria, demonstrating the ability of the bacteria to enter the cells.
From: Identification of bacteria-derived HLA-bound peptides in melanoma
a, Melanoma cells stained an anti-HLA antibody (red) were cocultured with anaerobically grown bacteria F. nucleatum or A. odontolyticus. These bacteria were labelled with click chemistry (green). Cell nuclei were stained with DAPI (blue). White arrows indicate the location of bacteria that entered the melanoma cells. b, A representative image of 51AL cells stained with the anti-HLA antibody (red) cocultured with F. nucleatum that were not grown with D-GalNAz and labelled with Alexa Fluor F488 (green), to exclude nonspecific staining. Images are presented at 63× magnification. Scale bars, 10 μm. Figures are representative of at least three independent experiments.
