Extended Data Fig. 11: CLEM images showing entry of bacteria into melanoma cells.
From: Identification of bacteria-derived HLA-bound peptides in melanoma
Fusobacterium nucleatum was grown with D-GalNAz and then labelled with DIBO–Alexa Fluor 488. Actinomyces odontolyticus and S. caprae were incubated with an anti-LTA antibody, and then with an anti-mouse secondary antibody labelled with Alexa Fluor 488. The 51AL and 55A3 cell lines were coincubated with the bacteria for 8 h. Ultra-thin sections were analysed by fluorescence microscopy to identify the bacteria (green and blue labelling are for bacteria and nucleus, respectively), followed by transmission electron microscopy (TEM) of the same cells for high-resolution morphology. Left and middle panels show CLEM and TEM images, respectively. Scale bars, 5 μm. The right panel shows high-magnification TEM image of the area in the black box in the corresponding middle panel. Scale bars, 1 μm. Bacteria that entered the melanoma cell are indicated with a white arrow. N, nucleus; M, mitochondrion; ER, endoplasmic reticulum. Figures are representative of at least three independent experiments.
