Extended Data Fig. 12: TIL reactivity towards bacteria-derived antigens.
From: Identification of bacteria-derived HLA-bound peptides in melanoma
Flow cytometry analysis of IFNγ-secreting TILs after coculture of 51AL and 55A3 TILs with B cells loaded with the indicated bacterial peptide. Each peptide was loaded on a different B cell that exhibited the HLA alleles to which the peptide was predicted to bind. TILs were stained with anti-IFNγ and anti-CD45 bifunctional antibody, which binds secreted IFNγ. The value indicates the ratio of IFNγ-secreting cells with the peptides to those with the DMSO control. Grey dots indicate the results of n = 3 biological replicates, and blue dots represent the average of replicates. Bars represent s.e. between replicates.
