Extended Data Fig. 6: scRNA-seq analysis of iBlastoid and blastocyst datasets.
From: Modelling human blastocysts by reprogramming fibroblasts into iBlastoids
a, Proportion of cells from each donor across all assigned clusters in the iBlastoid scRNA-seq dataset. b, Heat map showing gene expression profiles of each assigned cluster (top 10 genes each) in the iBlastoid scRNA-seq dataset. c, Unsupervised clustering of day 21 reprogrammed cells in the scRNA-seq dataset. d, Pearson correlation analysis for clusters from day 21 reprogrammed cells with annotated IM clusters from the iBlastoid scRNA-seq dataset. e, UMAP projection showing cell distribution of iBlastoids and blastocysts12,13 in the integrated dataset of iBlastoids and E5–7 blastocysts12,13. f, Expression of EPI markers (POU5F1 and NANOG), TE markers (CDX2 and GATA2), and PE markers (SOX17 and GATA6) for the integrated dataset. g, Per-cell expression score of EPI, TE, and PE signatures in the integrated dataset. h, PCA plot of pseudo-bulk RNA-seq data from scRNA-seq dataset of iBlastoids and blastocysts12,13 integrated with RNA-seq dataset of PS, TS and nEnd cell lines25,53. For more details on sample numbers, see ‘Statistics and Reproducibility’.
