Extended Data Fig. 7: Assessment of mural and polar TE gene expression in iBlastoid scRNA-seq dataset.
From: Modelling human blastocysts by reprogramming fibroblasts into iBlastoids
a, Unsupervised clustering of the integrated dataset with assigned cluster names. b, Proportion of cells for iBlastoid and human blastocyst datasets12,13 within each integrated cluster with respective original cell ID before integration analysis. c, Pearson correlation analysis of iBlastoid EPI, TE, and PE clusters with annotated EPI, TE, and PE clusters from blastocysts12,13. d, UMAP representation of E5–E7 TE cells of Petropoulos dataset13. e, f, Defined mural (e) and polar (f) TE signatures for the Petropoulos dataset13. g, Binned subtype scores for mural and polar TE signatures along UMAP component 1. h, Immunostaining of iBlastoid for CCR7; n = 4. Scale bar, 20 μm. i, CCR7 fluorescence intensity of polar and mural TE cells on iBlastoids; n = 4. Line, median; whiskers, maxima and minima; box limits, 25th to 75th percentiles. For more details on sample numbers, see ‘Statistics and Reproducibility’.
