Extended Data Fig. 8: Characterization of stem cells derived from iBlastoids.
From: Modelling human blastocysts by reprogramming fibroblasts into iBlastoids
a, Schematic of the experimental design for characterization of naive bPSCs, primed bPSCs and bTSCs. b, Immunostaining of naive bPSCs for KLF17 and NANOG, primed bPSCs for TRA160 and NANOG and bTSCs for KRT7 and GATA2, related to Fig. 3b–g; n = 2. c, qRT–PCR analysis of naive bPSCs for naive pluripotency markers, primed bPSCs for primed pluripotency markers and bTSCs for TSC markers; mean ± s.e.m., n = 4. d, e, EBs derived from #primed bPSCs (d) and primed bPSCs (e); n = 2. # indicates primed bPSCs generated from naive bPSCs. f, ScoreCard Assay analysis of EBs from #primed bPSCs and primed bPSCs; n = 2. g, h, Differentiation of #primed bPSCs (g) and primed bPSCs (h) into ectoderm, endoderm and mesoderm lineages; n = 2. i, Immunostaining of bTSC-differentiated EVTs for HLA-G; n = 2. j, Immunostaining of bTSC-differentiated STs for SDC1; n = 2. k, qRT–PCR analysis of bTSC-differentiated EVTs for EVT markers (ITGA1, ITGA5, FN1); mean ± s.e.m., n = 4. l, qRT–PCR analysis of bTSC-differentiated STs for ST markers (CSH1, SDC1, HSD3B1); mean ± s.e.m., n = 4. m, Fusion index of bTSC-differentiated STs; mean ± s.e.m., n = 12, two-tailed unpaired Student’s t-test. n, hCG protein level detected by hCG ELISA using conditioned medium collected from bTSC-differentiated STs; mean ± s.e.m., n = 2–3. Scale bars, 100 μm. For more details on sample numbers, see ‘Statistics and Reproducibility’.
