Extended Data Fig. 4: scRNA-seq pipeline and quality controls.
From: Modelling human blastocysts by reprogramming fibroblasts into iBlastoids
a, Phase-contrast images of the iBlastoids selected for scRNA-seq; n = 200. Scale bar, 100 μm. b, scRNA-seq analysis strategy (see Methods). c, UMAP representation of donor cell distribution for iBlastoids. d, UMAP representation of the cell cycle stages for iBlastoid scRNA-seq library. e, Expression of Sendai-KLF4, Sendai-KOS and Sendai-MYC in iBlastoids. f, Expression of GATA3 and KRT8 on UMAP of iBlastoid dataset. g, Expression of EPI markers (POU5F1 and NANOG), TE markers (CDX2 and GATA2), and PE markers (SOX17 and GATA6) for Petropoulos scRNA-seq library13. For more details on sample numbers, see ‘Statistics and Reproducibility’.
