Extended Data Fig. 7: Rec114–Mei4 and Mer2 form mixed condensates.

a, Rec114–Mei4 colocalizes with Mer2 in mixed condensates irrespective of DNA concentration. Reactions containing 16 nM Rec114–Mei4 and 100 nM Mer2 in the presence of 1, 10, or 100 ng μl⁻¹ plasmid DNA were assembled for 20 min at 30 °C. DAPI (5 μg ml⁻¹) was added to the reaction before application to glass slides. DNA enrichment within the condensates is visible at lower DNA concentrations (top, middle), but is not as clear at high DNA concentrations (bottom). The ratios of Rec114–Mei4 (heterotrimers) and Mer2 (tetramers) to each 2.6-kb plasmid DNA molecule are indicated on the right. Colocalization of Rec114–Mei4 and Mer2 complexes is evident even with a molar excess of DNA molecules, demonstrating that formation of joint foci is not simply because both protein complexes are independently associating with a limiting number of DNA substrates. b, Correlated intensity of Rec114–Mei4 and Mer2 proteins within the condensates. Each point shows the fluorescence intensity in an individual focus (n = 950, 925 and 1,000 foci from 2–3 fields of view for samples with 1, 10 and 100 ng μl⁻¹ DNA, respectively), normalized to the average focus intensity per field of view. The strong correlation indicates that the composition of the condensates is highly uniform between foci. In the presence of high DNA concentration, the fraction of smaller foci increased and correlated intensities decreased. c, Recruitment of soluble Rec114–Mei4 (left) or Mer2 (right) into preassembled condensates of Mer2 (left) or Rec114–Mei4 (right). Arrowheads, examples of the preassembled condensates. d, Pulldown of purified Mer2 on amylose resin with or without immobilized Rec114–Mei4 complexes. e, XL–MS of Rec114–Mei4–Mer2 condensates (620 crosslinked peptides, 229 distinct crosslinked pairs of lysines).