Extended Data Fig. 3: As a consequence of polytubey, multiple sperm cell pairs are released in ecs1 ecs2 mutant ovules.
From: Fertilized egg cells secrete endopeptidases to avoid polytubey
a, Three representative images showing two additional sperm cell pairs at 24 HAP, respectively. b–d, Time series showing representative images of two additional sperm cell pairs at 6 HAP (b), 8 HAP (c) and10 HAP (d), respectively. e, Proportions of additional sperm pairs in ovules of ecs1 ecs2 mutants after fertilization (n = 1056 for 6 HAP; 1114 for 8 HAP, 1083 for 10 HAP). Data are presented in box-and-whisker plots. Bottom and top of the box, 25th and 75th percentiles; centre line, 50th percentile; whiskers, minimum and maximum data. Abbreviations: sp., sperm cell; enn, endosperm nucleus; zyn, zygote nucleus. Scale bars, 20 μm.
