Extended Data Fig. 5: Truncated ECS1 and ECS2 proteins are not secreted from egg cells during fertilization.
From: Fertilized egg cells secrete endopeptidases to avoid polytubey
a, d, ECS1–GFP and ECS2–GFP were located inside the egg cell before fertilization. b, e, ECS1–GFP and ECS2–GFP were secreted from the egg cell at 8 HAP. Asterisks mark secreted ECS1–GFP and ECS2–GFP, respectively. c, f, Truncated TECS1–GFP (c) and TECS2–GFP (f) versions lacking signal peptides could not be secreted from the egg cell at 8 HAP. ec, egg cell. Dashed lines outline the egg cell boundaries. Insets show enlargements of regions indicated. Scale bars, 20 μm.
