Extended Data Fig. 5: COApm mediates suppression of mating behaviours towards unhealthy females.
a–d, These data are associated with Fig. 2a–e. a, Representative image of ChR2 expression in the COApm. b, Total direct investigation time of healthy females in the presence (ON) and absence (OFF) of COApm photoactivation. Duration of self grooming (c) and percentage of photoactivation trials with self grooming (d). Scale bar, 1 mm. e, f, These data are associated with Fig. 2i–m. Representative image of hM4Di expression in the COApm (e) and total direct investigation time of LPS females (f). Scale bar, 1 mm. g–k, Additional control experiments for data in Fig. 2i–m. Male mice expressing inhibitory DREADD (AAV2-hSyn-hM4D(Gi)-mCherry) in COApm were injected with either saline or CNO and tested for mounting behaviour towards LPS females (g). Percentage of male mounting (h), mounting time (i), number of mounts (j) and latency to mount (k) (saline, n = 8 and CNO, n = 7; from 2 independent experiments). l–p, Male mice expressing mCherry (AAV2-hSyn-mCherry) or inhibitory DREADD (hM4Di, AAV2-hSyn-hM4D(Gi)-mCherry) in COApm were injected with CNO and tested for mounting behaviour towards untreated, healthy oestrus females (l). Percentage of male mounting (m), mounting time (n), number of mounts (o) and latency to mount (p) (mCherry, n = 8 and hM4Di, n = 8; from 2 independent experiments). q–u, Male mice expressing inhibitory DREADD (AAV2-hSyn-hM4D(Gi)-mCherry) in COApm were injected with saline or CNO and tested for mounting behaviour towards untreated, healthy dioestrus females (q). Percentage of male mounting (r), mounting time (s), number of mounts (t), and latency to mount (u) (saline, n = 6 and CNO, n = 6; from 2 independent experiments). *P < 0.05, **P < 0.01 calculated by unpaired two-tailed t-test (c, i, j). Data are mean ± s.e.m. P values are described in the Supplementary Statistical Informatio.
