Extended Data Fig. 6: Differential gene expression in HFSCs of control, ADX and dermal GR-knockout mice.
From: Corticosterone inhibits GAS6 to govern hair follicle stem-cell quiescence
a, Sample clustering based on Pearson’s correlation of transcriptomes in HFSCs from sham, ADX, and ADX+CORT as well as control and Pdgfra-CreER;GRfl/fl mice. b, Principal component analysis (PCA) comparing the transcriptome of HFSCs from sham, ADX, ADX+CORT, control and Pdgfra-CreER;GRfl/fl mice. c, Heat map of log2-transformed fold change of gene expression of 121 common genes among ADX (versus sham), Pdgfra-CreER;GRfl/fl (versus control), and ADX+CORT (versus ADX). Cell-cycle-related genes are noted in orange. d, Left, heat map of log2-transformed fold change of gene expression of transcription factors (Foxc1, Lhx2, Foxp1, Nfatc1), key signalling factors (Fgf18), or downstream readout of key signalling factors (Id1 for BMP pathway, Axin2 for WNT pathway, Gli1 for SHH pathway) known to regulate HFSC quiescence. Right, heat map of log2-transformed fold change of gene expression of 7 core genes related to cell-cycle machineries and cytokinesis. e, f, RT–qPCR of genes related to cell-cycle machineries and cytokinesis from telogen HFSCs of sham and ADX mice (e) and control and Pdgfra-CreER;GRfl/fl mice (f). Data are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001. For exact P values, see Source Data. For statistics, sample sizes and numbers of replications, see Methods.
