Extended Data Fig. 7: The expression of cell-cycle-related genes in HFSCs.
From: Corticosterone inhibits GAS6 to govern hair follicle stem-cell quiescence
a, b, RT–qPCR of genes related to cell-cycle machineries and cytokinesis using telogen HFSCs from vehicle and corticosterone-fed mice (a) and control and stressed mice (b). c, d, RT–qPCR of genes related to cell-cycle machineries and cytokinesis in telogen epidermis from sham and ADX mice (c) and vehicle and corticosterone-fed mice (d). e, Experimental workflow of the differentially expressed genes (DEGs, >1.5-fold, Padj < 0.05) from DP cells of sham and ADX mice, as well as control and Pdgfra-CreER;GRfl/fl mice. f, g, Immunohistochemical analysis (PCAD) of skin samples from sham and ADX (f) or control and Pdgfra-CreER;GRfl/fl (g) mice used in RNA-seq experiments to validate hair cycle (all telogen). Dashed lines, epidermis and hair follicles. h, Top, FACS strategies for isolating DP cells for RNA-seq24,44. Bottom, the expression levels of cell-type-specific signature genes (DP, fibroblasts, HFSCs and mast cells) in FACS-purified DP cells. TPM, transcripts per million. i, Sample clustering based on Pearson’s correlation of transcriptomes in DP of sham and ADX mice (left), as well as control and Pdgfra-CreER;GRfl/fl mice (right). j, Heat maps of the differentially expressed genes (DEGs, > 1.5-fold, Padj < 0.05) from FACS-purified DP cells of sham and ADX mice (left) or control and Pdgfra-CreER;GRfl/fl mice (right). Scale bars (f, g), 50 μm. Data are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, NS, not significant. For exact P values, see Source Data. For statistics, sample sizes and numbers of replications, see Methods.
