Extended Data Fig. 8: Transcriptome analysis and secretome analysis identified GAS6 as a secreted factor suppressed by systemic corticosterone in the DP.
From: Corticosterone inhibits GAS6 to govern hair follicle stem-cell quiescence
a, Secretome analysis identifying common secreted factors from DEGs (>1.5-fold, Padj < 0.05) in ADX and Pdgfra-CreER;GRfl/fl DP cells identified by RNA-seq. b, c, Expression levels of shared differentially expressed secreted factors as TPM, in the DP cells of ADX (b) and Pdgfra-CreER;GRfl/fl (c) mice. d, Top, negative control and Gas6 mRNA expression by in situ hybridization in late anagen (AnaV) and mid catagen (CatV) skin of sham and ADX mice. Bottom, quantification of Gas6 mRNA in the DP. Dashed lines, hair follicle; solid lines, DP. e, Representative image of negative control and Axl mRNA expression by in situ hybridization in telogen skin. Yellow dashed lines: bulge; white dashed lines: hair germ (top). RT–qPCR of Axl, Tgfbr1, Bmpr1a, Nfatc1 and PPIB from HFSCs and epidermal stem cells (EpSCs) of control mice (P83) (bottom). f, Representative images of negative control and Axl mRNA expression by in situ hybridization in late anagen skin. Yellow dashed lines: bulge; white dashed lines: epidermis and hair follicles. g, The expression levels (as TPM) of genes encoding TAM receptors (Tyro3, Axl and Mertk) in HFSCs. h, Left, schematic of the GAS6–AXL receptor tyrosine kinase pathway. R428 is a selective inhibitor of AXL tyrosine kinase activity43. Right, colony-formation assays of cultured HFSCs in R428 or GAS6 with R428 with quantifications. Scale bars (d–f), 50 μm. Data are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, NS, not significant. For exact P values, see Source Data. For statistics, sample sizes and numbers of replications, see Methods.
