Extended Data Fig. 3: Removal of the adrenal glands in stressed or aged mice leads to hair follicle regeneration.
From: Corticosterone inhibits GAS6 to govern hair follicle stem-cell quiescence
a, Plasma corticosterone levels at P62 in non-stressed control and stressed mice. b, c, H&E staining (b) and immunohistochemical analyses (active caspase3 (aCAS3) and PCAD) (c) in control and stressed mice. Dashed lines, epidermis and hair follicles. d, Stressed sham (sham+stress) and stressed ADX (ADX+stress) mice were monitored for hair coat recovery. Quantification shows the percentage of back skin that is covered by newly regenerated hairs. e, Plasma levels of corticosterone in young mice (P46, P77, and P98) and aged mice (P427 and P581). f, Sham and ADX operations were performed on aged mice (P521). The mice were shaved and monitored for hair coat recovery from P521 to P574. Scale bars (b, c), 50 μm. Data are mean ± s.e.m. *P < 0.05, **P < 0.01, ****P < 0.0001. For exact P values, see Source Data. For statistics, sample sizes and numbers of replications, see Methods.
