Extended Data Fig. 4: GR depletion in different cell types in the skin.

a, Top, K15-CrePGR depletes GR efficiently in HFSCs. Bottom, immunohistochemical analysis (GR and CD140a) of telogen hair follicle in the skin of control and K15-CrePGR;GR^fl/fl mice. b, Hair cycle progression of control and K15-CrePGR;GR^fl/fl mice. c, Immunohistochemical analyses (GR and PCAD) of telogen hair follicles in the skin of control and Pdgfra-CreER;GR^fl/fl mice, showing that Pdgfra-CreER depletes GR efficiently in the dermal fibroblasts and DP. d, Immunocolocalization (EdU and CD34) in control and Pdgfra-CreER;GR^fl/fl hair follicles after tamoxifen administration. EdU incorporation reveals premature HFSC activation in the hair follicles of Pdgfra-CreER;GR^fl/fl mice. e, Comparison of EdU localization in bulge and upper ORS in late anagen (AnaV) of control (P124) and Pdgfra-CreER;GR^fl/fl (P73) mice. f, Representative hair regeneration status of control and Pdgfra-CreER;GR^fl/fl mice from P73 to P205, with quantification of the number of hair cycles. g, Immunocolocalization (EdU and CD34) in infundibulum, junctional zone, sebaceous gland, mid ORS, lower ORS, and matrix of late anagen (AnaVI) hair follicles in control and Pdgfra-CreER;GR^fl/fl mice during late anagen with quantifications. h, Top, H&E staining in the late anagen skin of control and Pdgfra-CreER;GR^fl/fl mice, with quantification of the thickness of epidermis (E). Bottom, immunocolocalization (EdU and DAPI) in interfollicular epidermis and dermis in control and Pdgfra-CreER;GR^fl/fl mice. Scale bars (a, c–e, g, h), 50 μm. Yellow dashed lines, bulge (a, c–e); white dashed lines, hair germ (a, c, d), the rest of hair follicles (e, g), or the boundary between the epidermis and the dermis (h); solid white line, DP (a, c, d). Data are mean ± s.e.m. **P < 0.01, ***P < 0.001, ****P < 0.0001, NS, not significant. For exact P values, see Source Data. For statistics, sample sizes and numbers of replications, see Methods.

Source data