Extended Data Fig. 5: Corticosterone acts on the DP.
From: Corticosterone inhibits GAS6 to govern hair follicle stem-cell quiescence
a, RT–qPCR of GR from DP and DF. b, Immunohistochemical analyses (YFP and DAPI) of anagen skin from Sox2-CreER;R26-lsl-YFP mice. Left, the arrowhead indicates an anagen guard hair follicle with YFP+ DP cells. Right, quantification of the percentage of YFP+ and YFP− DP in Sox2-CreER;R26-lsl-YFP. Only guard hair follicles have YFP+ DP. c, Immunohistochemical analyses (GR and DAPI) of skin from control and Sox2-CreER;GRfl/fl mice. Dashed lines, epidermis and hair follicles; solid line, DP. The arrowhead indicates the DP of Sox2-CreER;GRfl/fl guard hairs. d, Representative hair regeneration status of control and Sox2-CreER;GR fl/fl mice from P45 to P160. Quantification shows the number of hair cycles for guard hairs and other hairs in control and Sox2-CreER;GRfl/fl mice. e, Comparison of the hair bulb diameter in late anagen (AnaV) in the skin of control (P120) and Sox2-CreER;GRfl/fl (P67) mice. Yellow lines indicate the hair bulb diameter. The arrowhead denotes minor hyper thickening of the Sox2-CreER;GRfl/fl hair follicle around the ORS, probably because the dermis has not expanded to accommodate the extra proliferation from HFSCs. f, Immunocolocalization of phosphohistone H3 (pHH3) and CD34 in bulge and upper ORS, middle ORS, lower ORS and matrix of late anagen (AnaV) guard hair follicles in control and Sox2-CreER;GRfl/fl mice. The white arrowhead denotes a thickened region in a Sox2-CreER;GRfl/fl hair follicle, probably due to excessive proliferation from HFSCs. Yellow dashed lines, bulge; white dashed lines, the rest of the hair follicle. g, Hair shaft length of guard hairs in control and Sox2-CreER;GRfl/fl mice after anagen. Scale bars, 50 μm (b, c, e, f), 1 mm (d, g). Data are mean ± s.e.m. ****P < 0.0001, NS, not significant. For exact P values, see Source Data. For statistics, sample sizes and numbers of replications, see Methods.
