Fig. 2: Sequence, structure and epigenetic map of the chromosome 8 centromeric region.
From: The structure, function and evolution of a complete human chromosome 8
a, Schematic showing the composition of the CHM13 chromosome 8 centromere. The centromeric region consists of a 2.08-Mb D8Z2 α-satellite HOR array flanked by regions of monomeric and/or divergent α-satellite interspersed with retrotransposons, β-satellite and γ-satellite. The predicted restriction digest pattern is shown. The D8Z2 α-satellite HOR array is heavily methylated except for a 73-kb hypomethylated region, which is contained within a 632-kb CENP-A chromatin domain (Extended Data Fig. 9, Supplementary Fig. 8). A pairwise sequence identity heat map indicates that the centromere is composed of five distinct evolutionary layers (dashed arrows). b, Pulsed-field gel Southern blot of CHM13 DNA confirms the structure and organization of the chromosome 8 centromeric HOR array. Left, ethidium bromide (EtBr) staining; right, 32P-labelled chromosome 8 α-satellite-specific probe. n = 2. See Supplementary Fig. 9a, b for gel source data. c, Representative images of a CHM13 chromatin fibre showing CENP-A enrichment in an unmethylated region. n = 3. Scale bar, 1 μm.
