Extended Data Fig. 10: TCPOBOP protection against bile acid-induced ileitis requires CAR expression in T cells.
From: CAR directs T cell adaptation to bile acids in the small intestine
a, Mean ± s.e.m. weight loss (n = 5 per group) of co-housed B6.Rag2−/− mice transplanted with CAR-deficient (B6.Nr1i3−/−) CD4+ naive T cells and maintained on a cholic acid-supplemented diet with (CA/TC) or without (CA/Veh) TC treatment. Weights are shown relative to three weeks after transfer, when TC treatments were initiated. NS, not significant; two-way ANOVA. b, H&E-stained sections of terminal ilea or colons from B6.Rag2−/− mice reconstituted with CAR-deficient T cells and treated as above and as indicated. Representative of five mice per group analysed in two independent experiments. Magnification, 10×; scale bars, 100 μm. c, Mean ± s.e.m. histology scores for colons and terminal ilea as in b. NS, not significant by unpaired two-tailed Student’s t-test.
