Extended Data Fig. 2: Inhibition of bile acid reabsorption rescues ileitis induced by CAR-deficient T cells in reconstituted Rag−/− mice.
From: CAR directs T cell adaptation to bile acids in the small intestine
a, Mean ± s.e.m. weight loss of co-housed B6.Rag2−/− mice transplanted with wild-type (B6; blue; n = 15) or CAR-deficient (B6.Nr1i3−/−; red; n = 13) naive CD4+ T cells and treated with 2% (w:w) cholestyramine (CME) beginning three weeks after T cell transfer. NS, not significant by two-way ANOVA. b, Top, H&E-stained sections of colons or terminal ilea from B6.Rag2−/− mice reconstituted with wild-type or CAR-deficient T cells and treated with or without CME as in a. Representative of 12 mice per group analysed in 3 independent experiments. Magnification, 10×; scale bars, 100 μm. Bottom, mean ± s.e.m. histology scores (n = 12) for colons or terminal ilea as in a. NS, not significant by unpaired two-tailed Student’s t-test. c, Mean ± s.e.m. weight loss (n = 16 per group) of co-housed B6.Rag1−/− mice with or without ASBT (encoded by Slc10a2) after reconstitution with wild-type (B6) or CAR-deficient (B6.Nr1i3−/−) naive CD4+ T cells. **P = 0.005 (CAR-deficient T cell transfer into control versus Slc10a2−/−Rag1−/− recipients), two-way ANOVA. d, Top, H&E-stained colon or terminal ileum sections from control or ASBT-deficient B6.Rag1−/− mice reconstituted with wild-type or CAR-deficient T cells as in c. Representative of five mice per group. Bottom, mean ± s.e.m. histology scores for colons or terminal ilea (n = 5) as above. P values (one-way ANOVA with Tukey’s correction for multiple comparisons) are listed. NS, not significant.
