Extended Data Fig. 3: In vivo 2-NBDG uptake does not mirror FDG uptake.
From: Cell-programmed nutrient partitioning in the tumour microenvironment
a, Representative histograms of in vivo 2-NBDG uptake in splenic and MC38 tumour cell subsets. b, MFI of in vivo 2-NBDG uptake across spleen and MC38 tumour cells (n = 3 mice). c, d, Representative histograms of 2-NBDG uptake in vivo in splenic CD4+ (c) and CD8+ (d) T cells. e, 2-NBDG staining in splenic CD4+ and CD8+ subsets (n = 3 mice). CM, central memory T cell; EM, effector memory T cell; N, naive T cell; Tconv, conventional CD4+ T cell; Treg, regulatory CD4+ T cell. f, Schema for 2-NBDG and FDG co-injection experiment. g, Representative histogram of 2-NBDGhi and 2-NBDGlo populations collected through flow sorting. h, Per-cell FDG avidity of flow-sorted 2-NBDGlo versus 2-NBDGho splenic T cells (n = 3 mice). Each data point represents a biological replicate; data are mean ± s.e.m. Data are from representative studies performed independently at least twice. P values were calculated using the Brown–Forsythe and Welch ANOVA with Dunnett’s T3 for multiple comparison tests (b, e), two-tailed Welch’s t-test for CD4+ comparisons (e) or a paired t-test (h); *P < 0.05, **P < 0.01, ***P < 0.001; exact P values are provided in the Source Data.
