Extended Data Fig. 5: Tumour model characterizations by flow cytometry.
From: Cell-programmed nutrient partitioning in the tumour microenvironment
a–g, Spleen and tumour CD45+ immune cell populations from MC38 (a; n = 3 mice), CT26 (b; n = 4 mice) and Renca (c; n = 4 mice) subcutaneous tumours; intrarenal Renca tumours (d; n = 3 mice); spontaneous PyMT GEMM tumours (e; n = 3 mice); AOM/DSS CRC tumours (f; n = 6 mice for tumours; n = 11 mice for spleens); and MC38 subcutaneous tumours grown in Rag1−/− mice (g; (n = 6 mice). DC, dendritic cell; NK cell: natural killer cell, PMN-MDSC, polymorphonuclear myeloid-derived suppressor cell. h, Gating strategy for immune cell identification using lymphocyte and myeloid-focused antibody panels. Each data point represents a biological replicate; data are mean ± s.e.m. Data in a–f are representative of independent experiments performed at least twice.
