Extended Data Fig. 2: SDS–PAGE (12.5%) of recombinant citrate synthases from D. acetivorans after purification with Ni-NTA column.
From: High CO2 levels drive the TCA cycle backwards towards autotrophy
M, molecular mass standard proteins; lane 1, Desace_08345 (2 μg); lane 2, Desace_06860 (2 μg); lane 3, Desace_09325 (2 μg). Their predicted molecular masses are 49 kDa (Desace_08345) and 50 kDa (Desace_06860; Desace_09325). Proteins were stained with Coomassie blue. For gel source data, see Supplementary Fig. 1. The SDS–PAGE analysis was conducted three times and all results were similar to the ones shown here.
