Extended Data Fig. 3: Protein quantification using MS.
From: High CO2 levels drive the TCA cycle backwards towards autotrophy
a, CLUSTAL format alignment by MAFFT (v.7.452) of sequence parts of citrate synthase isoforms to illustrate the unique peptides chosen for quantification. Desace_06, Desace_06860; Desace_08, Desace_08345; Desace_09, Desace_09325. b, Calibration curves generated for each citrate synthase using tryptic digests of the recombinant proteins isolated by one-dimensional PAGE. Injection volume of standard solution (μl, y axis) versus MS peak area in MRM experiments (x axis) as calculated by Skyline.
