Extended Data Fig. 8: Mapping cellular interactions between SARS-CoV2-infected and non-infected compartments.

a, Example description of the derivation of a region adjacency graph for a given lung IMC image. Left, DNA channel that marks nuclei; middle, identified metaclusters; and right, region adjacency graph represented as edges between adjacent cells. Scale bar, 100 μm. b, Observed values of pairwise cluster interactions over the expected values for the same cellular interactions for the image in a. c, Pairwise interactions between metaclusters, aggregated by the mean value across images depending on the disease group. d–f, Pairwise cellular interactions between metaclusters, depending on SARS-CoV-2 S positivity in uninfected cells (d), between SARS-CoV-2 S⁺ and S⁻ cells (e) and between infected cells (f). g–i, Statistical testing of differential interactions of infected cells and other cell types, and uninfected cells and other cell types, depending on SARS-CoV-2 S positivity of the second cell type. g, Both SARS-CoV-2 S⁻ and S⁺ cells. h, Only SARS-CoV-2 S⁺ cells. i, Only SARS-CoV-2 S⁻ cells. The rows display a volcano plot in which the x axis displays the difference in interaction between SARS-CoV-2 S⁺ and S⁻ cells and the y axis displays the −log₁₀-transformed Mann–Whitney U-test, FDR-adjusted P value.