Extended Data Fig. 9: Expression of TMEM16A, TMEM16F and ACE2 in different experimental conditions.

a, Levels of expression of the genes investigated in this study upon their individual knockdown in Vero, HEK293/ACE2 and Calu-3 cells. RNA was prepared from the different cell lines after transfection with the indicated siRNAs (siNT1-control non-targeting siRNA1) and mRNA levels were quantified by qPCR. Data are expressed as fold change compared with mock-treated cells after normalized over the GAPDH housekeeping mRNA. Data are mean ± s.e.m. of three independent experiments. b, Western blotting analysis showing the levels of ACE2 and TMEM16F in the presence of spike protein after silencing selected genes (ACE2, TMPRSS2, TMEM16A, TMEM16B, TMEM16E, TMEM16F and XKR8). The amount of spike, ACE2 and TMEM16F measured by immunoblotting is shown after cell treatment with the respective siRNAs. The levels of spike, ACE2 and TMEM16F proteins were assessed by immunoblotting with anti-V5, anti-ACE2 and anti-TMEM16F antibodies respectively. β-actin was used as a loading control. Representative blot of three independent repetitions. c, As in b in HEK293/ACE2 cells. The blot with the anti-TMEM16A antibody did not detect any band of the expected mass (Extended Data Fig. 7b).