Extended Data Fig. 4: Analysis of screening results.
From: Drugs that inhibit TMEM16 proteins block SARS-CoV-2 spike-induced syncytia
a, Effect of individual drugs in the SIA screening grouped according to therapeutic classes. Data are z-scores from the screening of both libraries (drugs present in both libraries are duplicated). The dotted lines are at z-scores ± 2.58 (red) and ± 1.96 (blue). Antipsychotics, antidepressants and H1 histamine receptor antagonists exerted negative effects on syncytia formation. Another inhibitory drug class included cardiac glycosides, as digitoxin, ouabain, lanatoside C and digitoxigenin were all in the 0.025% tail of the distribution when used at 10 μM. On the contrary, antifungal drugs of the imidazole class had a trend to increase the frequency of syncytia. b, Venn diagram showing the drugs with z-scores <−2.58 from the results of the CFIA and SIA screenings using the two drug libraries, as indicated. Drugs in bold are those further tested on SARS-CoV-2 infection. CFIA and SIA are intrinsically different, as the former tests for plasma membrane fusion events between single, heterologous cells while the latter scores for the formation of larger syncytia.
