Extended Data Fig. 7: CNMaR-expressing neurons are located in the anterior midgut and the brain and are not overlapped with Ir76b+ neurons, and some respond to amino acid deprivation.
From: Response of the microbiome–gut–brain axis in Drosophila to amino acid deficit
a, b, Representative confocal images of the anterior midgut of an adult fly carrying CNMaR-Gal4 and UAS-CD8-GFP that were co-labelled with anti-GFP antibody (green) and DAPI (blue) (a), and the brain of an adult fly carrying Ir76b-Gal4>UAS-CD8-GFP and CNMaR-LexA>LexAop-tdTomato, probed with anti-GFP (green) and anti-DsRed (red) antibodies, illustrate two populations of neuronal processes: one from Ir76b+ taste sensory neurons and another from CNMaR+ central nervous system (CNS) neurons (b). c, Measurements of calcium mobilization by CaLexA reporter (green) in mated female flies carrying CNMaR-Gal4>UAS-CaLexA (flies of around 2 days old maintained for 5 days on a high-amino-acid diet containing 10% dietary yeast) that were subjected to amino acid deprivation by transferring half of the flies to an agar medium containing 100mM d-glucose only for 48 h (right, protein deprivation) or that continued on the high-amino-acid diet (left, no deprivation). Arrowheads denote the enhancement of GFP intensity in the ellipsoid body (right). Scale bars, 200 μm (a); 50 μm (b, c). Z-stacked projection images are shown. Representative images were selected from at least five independent samples collected in each experiment.
