Extended Data Fig. 9: Acetobacter colonization results in a significant increase in the amino acid absorption of the host and the suppression of the l-EAA preference through CNMaR-expressing neurons.
From: Response of the microbiome–gut–brain axis in Drosophila to amino acid deficit
a, Levels of ingested deuterium-labelled amino acids (l-leucine-5,5,5-d3, l-phenyl-d5-alanine-2,3,3-d3 or l-glutamic acid-2,3,3,4,4-d5) in the haemolymph of GF control flies (−) fed on 5% sucrose alone and in the haemolymph of treated GF flies (+) fed on 5% sucrose containing deuterium-labelled amino acids (n = 3). b, Two-choice preferences of GF flies or GF flies that had been mono-associated with A. pomorum in which CNMaR+ neurons were conditionally activated by the heat-inducible TrpA1 at 30 °C. Note that a significantly higher bacterial load (approximately 10 times higher number of colony-forming units) of intestinal A. pomorum was found in flies maintained at 30 °C than in flies maintained at 22 °C in which control experiments were conducted (data not shown). GF-Aceto, GF flies mono-associated with A. pomorum; GF-Lacto, GF flies mono-associated with L. plantarum WJL. Data are mean ± s.e.m. P values are indicated; one-way ANOVA with Tukey’s post-hoc test in a, b. Sample sizes and statistical analyses are shown in Supplementary Table 1.
