Extended Data Fig. 6: Enhancement of the CNMa promoter activity by co-expression of Atf4 and Mitf.
From: Response of the microbiome–gut–brain axis in Drosophila to amino acid deficit
The 5′-flanking region (−1573/+1) of the CNMa gene was used to generate a pGL3-CNMa reporter plasmid. Drosophila S2 cells were transfected with the pGL3-CNMa reporter plasmid with pPac-Atf4 (Atf4), pPac-Mitf (Mitf) or both (Atf4 + Mitf). Luciferase activities were measured at 48 h after transfection. The luciferase activity of the pGL3-CNMa reporter co-transfected with an empty pPac vector was arbitrarily set to 1. The relative luciferase activities are shown as the mean ± s.e.m. from three independent experiments. P values are indicated (one-way ANOVA with Tukey’s post-hoc test). Statistical analyses are shown in Supplementary Table 1.
