Extended Data Fig. 7: Functional antibody profiling by systems serology.
From: Adjuvanting a subunit COVID-19 vaccine to induce protective immunity
a–c, SARS-CoV-2 spike-specific binding IgM (a), IgG1 (b) and IgA (c) responses in sera collected at days −7, 21 and 42. d, e, FcR-binding antibody responses FcR3A (d) and antibody-dependent neutrophil phagocytosis (ADNP) (e) measured in serum collected at days −7, 21 and 42. f, PLSDA analysis of all antibody features measured using systems serology. g, The top 3 antibody features discriminating protected vs. infected animals on day 42 in the PLSDA analysis. h, Heat map showing Spearman’s correlation between peak nasal viral load (left) or pharyngeal vial load (right) and antibody responses (day 42) indicated on the y axis. i, Heat map showing Spearman’s correlation between peak nasal viral load and antibody responses within each group. In a–e, the boxes show median, 25th and 75th percentiles and the whiskers show the range. Asterisks represent statistically significant difference between two groups determined by two-sided Mann–Whitney rank-sum test. In h, i, the P values were calculated for Spearman’s correlation. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
