Extended Data Fig. 6: Intestinal CX3CR1+ DCs migrate to the thymus.
a, Thymic DCs were analysed at two weeks after weaning for XCR1 and SIRPα. Flow cytometry of total DCs, CX3CR1+ DCs, CD103+ DCs and pDCs (n = 5). b–f, Caeca from KikGR33 transgenic mice were exposed to 405-nm-wavelength light or left untreated at 5 (young) or 14 (adult) weeks of age and two days later the thymus, MLNs and spleen were collected. b, Flow cytometry of RFP+MHCII+ cells in KikGR33 mice without light exposure (n = 5). c, Frequencies and counts of RFP+MHCII+ cells in young mice. d, e, Flow cytometry (d) and counts (e) of RFP+MHCII+ cells in adult mice. f, Flow cytometry of RFP+MHCII+ cells subsets in young and adult thymi. In c–f, n = 5 (young); n = 9 (adult). g–i, Mice were analysed at weaning (young) or at 12 weeks of age (adult) and thymus chemokine gene expression or colon lamina propria DC populations were analysed. g, Relative expression of chemokines in the thymus (n = 5 (young); n = 15 (adult)). h, CCR5+ cell frequencies of CX3CR1+ DCs in the colon (n = 10 per group). Wild-type and CX3CR1GFP/GFP (KO) mice were colonized with SFB at weaning and treated with TAK-779 or anti-CCL2 antibody and thymi were compared one week later. i, SFB-specific qPCR (n = 17 (WT); n = 13 (anti-CCR5 + anti-CCR2); n = 5 (WT anti-CCR2); n = 10 (KO); n = 5 (KO anti-CCR5 + anti-CCR2)). Each replicate is a biologically independent sample. Data are shown as individual values and mean; P values by two-tailed unpaired t-test (g, h) or one-way ANOVA with Fisher’s LSD post hoc test (i).
