Extended Data Fig. 2: Conditional ablation of GINIP⁺ neurons in GINIP–DTR mice and skin histopathological analysis after UV exposure.

a, Representative confocal images for DAPI (blue) and GINIP (green) staining of C3 DRGs from control DTR-control (top) and GINIP-DTR (bottom) mice, 10 weeks after diphtheria toxin (DT)-treatment. b, Absolute number of GINIP⁺, CGRP⁺, TAFA4⁺, TH⁺ and IB4⁺ DRG neurons were quantified in DTR-control (blue) and GINIP-DTR (red) mice (n = 4–6 independent DRG per group). c, Representative confocal images for Beta3-tubulin (blue) and GINIP (green) staining of mouse ear skin sections as in a. d, Scratching episodes were monitored for 30 min at each time point post-irradiation indicated (n = 6–10 mice per group). e, Representative H&E staining of ears from DTR (left) and GINIP-DTR (right) mice at D35 post-irradiation. f–i, Histopathological analysis for leukocyte infiltration (f), epidermal thickness (g), fibrosis (h) and fibrosis extension (i) (n = 5 mice per group); related to Fig. 1g–j. Criteria used for the histopathological scoring are described in the Methods. j, Representative Masson trichrome (top) and H&E staining (bottom) of back skin from DTR-control (left) and GINIP-DTR (right) mice at D35 post-irradiation. All data are representative of at least two independent experiments and presented as mean ± s.e.m. P values determined by one-way ANOVA with Tukey’s multiple comparisons test. NS, not significant; *P < 0.05; **P < 0.01; ***P < 0.001.

Source data