Extended Data Fig. 8: Analysis of mitochondria–ER contact site candidates.

a, Heat map of genes with a primary localization at the mitochondrial outer membrane and ER membrane/nuclear outer membrane and a secondary localization to the other compartment as computed by NMF. To be included on the heat map, genes required an NMF score of at least 0.15 in the compartments of interest, a score ratio of at least 0.4 between the primary and secondary localization, and a score ratio of at least 2 between the compartments of interest and all other compartments. Bold genes indicate those selected for mitochondrial morphology assays in the following panels. A grey dot on the right side of the plot indicates proteins involved in lipid and cholesterol homeostasis, and a pink dot indicates calcium signalling. b, Dot plot view of BioID data for mito–ER contact site candidates highlighting recovery of mitochondrial fission machinery, mito–ER tethers and outer mitochondrial membrane proteins. Asterisks on the heat map indicate spectral counts for prey genes corresponding to the bait that were ignored by SAINT as peptides from the bait confound accurately evaluating the abundance of itself as an interactor. c, Mitochondrial morphology is altered by transient expression of GFP-tagged CHMP7 and C18orf32, as monitored by confocal immunofluorescence microscopy in HeLa cells. Cells were fixed and probed with antibodies directed against GFP and COXIV (Methods). The white box indicates the zoomed area displayed in the rightmost panels. Scale bars, 10 μm.