Extended Data Fig. 9: Additional chemogenetic manipulations of ITCdm and ITCvm clusters.
From: Intercalated amygdala clusters orchestrate a switch in fear state
a, Chemogenetic manipulations. AAV encoding Cre-dependent hM3Dq was targeted to ITCdm neurons in Foxp2-Cre mice. b, ITCdm activation. Freezing on retrieval: Cre+, 45.7 ± 7.6%; Cre−, 28.5 ± 3.3%; *P = 0.0044. c, Schematic showing the dual-cluster chemogenetic manipulation experiment. An AAV encoding Cre-dependent KORD was targeted to both ITCvm and ITCdm neurons in Foxp2-Cre mice. d, Freezing behaviour of experimental (Cre+) and control (Cre−) mice; controls were injected with the AAV and administered the ligand. Arrows and colour-shadings indicate the timing of SalB administration before extinction retrieval. Freezing levels on extinction retrieval (day 3): Cre+, 46.4 ± 4.3%; Cre−, 27.3 ± 2.7%; *P < 0.0001, repeated-measures ANOVA followed by Sidak post hoc test. N.S., not significantly different on day 1 or day 2 freezing. Mean ± s.e.m. e, Examples of histological validation of KORD–mCitrine expression in ITCvm and ITCdm neurons across multiple anterior-to-posterior coronal sections. Following behavioural experiments, mice were killed and slices (50 μm) cut and stained with an anti-FOXP2 antibody. Scale bar, 200 μm. f, Heatmaps illustrating virus expression aggregated across Cre+ mice. Scale bar indicates the fraction of animals exhibiting expression at each locus (0: no mice expressed; 1: all mice expressed).
